Details of the single nucleotide polymorphisms (SNPs)
| SNP (location) | NCBI SNP Cluster ID* | Allele 1/ allele 2 | Primers | T (°C) |
|---|---|---|---|---|
| *From the clone gi:22044576 (http://www.ncbi.nlm.nih.gov/). Markers are ordered from pter to qter along chromosome 3. | ||||
| Details of the SNPs may be found in the dbSNP database under their respective Ids. | ||||
| F, Forward primer sequence (5′–3′); R, Reverse primer sequence (5′–3′); SS, SNaPshot primer with the length of tail described in subscript; T, annealing temperature (°C) for the polymerase chain reaction. | ||||
| SNP 1 (intron 1) | rs989692 | C/T | F: TCCCCCTTACTCCCCTCTTA | 61.8 |
| R: GCAAAACGAATGAACACATGA | ||||
| SS1 F: T37AATAGATACTGGTATCAGTAG | ||||
| SNP 2 (intron 4) | rs2196521 | C/T | F: AAAGCCTACCATCAGGGACA | 58 |
| R: CATGGACCACGCATTGAATA | ||||
| SS2 R: T19CTCACCTCTGATTAATCCCTT | ||||
| SNP 3 (intron 4) | rs1025192 | C/T | F: CACTCCATTTTCCAATGTGCT | 58 |
| R: TCCACTGTCGGACTTATCACC | ||||
| SS3 F: C26TCCACTATAAAAAATTCATC | ||||
| SNP 4 (intron 9) | rs1816558 | C/T | F: GTAGAGACGGGGTTTCACCA | 58 |
| R: AACCAACACCTTTCCCCAAT | ||||
| SS4 R: C43AAAACTAATAATCTTACAC | ||||
| SNP 5 (intron 11) | rs3773885 | C/T | F: CTTTACACACATTTCCATTTTTGC | 58 |
| R: CCTAGCATAATTAAGCCAACCATT | ||||
| SS5 R: T59AAGCTTAAAGGCTTTGTTTTC | ||||
| SNP 6 (intron 18) | rs3773882 | C/T | F: GCTCCAAGGGGTTTAGAAAGTT | 61.8 |
| R: GCCTTCCCAAGAAGAAGAAAAT | ||||
| SS6 R: C30ATCAAAAAAGAAAAAATTTC | ||||
| SNP 7 (intron 19) | rs3736187 | G/A | F: TCTGTGATGAGGAGGGATGAC | 58 |
| R: GATACACCATGCACTGGGATT | ||||
| SS7 R: C54TTTTACTTAAATAAATATATTA | ||||