Table 4

Neurodegeneration and iron density in deep grey matter (DGM) lesions in patients with multiple sclerosis (MS) and controls

DGM controlsMS NADGMMS inactive DGM lesionsMS active DGM lesions
APP-reactive neuronal cell bodies/mm2Median00.301.3
APP-reactive axonal spheroids/mm2Median03*5.0*79.8*†
E06-reactive neurons in %Median13.4869.10*84.49*84–84*
E06-reactive axonal spheroids/mm2Median0.31.40.52
E06-reactive thin cellular processes/mm2Median1.
E06-reactive oligodendrocytes/mm2Median1.715.2*12.6 *19.8
E06-reactive lipofuscin/mm2Median19.429.216.621.3
E06-positive area in %Median0.33.5*4.6*3.7
Oxidised DNA-reactive nuclei/mm2Median1.
Iron densityMedian612.3654.5469.4†546.7
  • This table depicts an overview of the extent of axonal injury/neurodegeneration in the DGM of patients with MS that was separated according to active lesions, inactive lesions, normal-appearing deep grey matter (NADGM) and control DGM.

  • In the NADGM, APP-positive axonal spheroids (p=0.001), E06-reactive neurons (p=0.001) and E06-reactive oligodendrocytes (p=0.014) were significantly increased compared with controls. The NADGM also displayed a higher percentage of E06-reactive areas (p=0.002). In the active and inactive lesions of the DGM, the levels of APP-positive axonal spheroids (p=0.003 and p<0.001, respectively) and E06-reactive neurons (p=0.024 and p<0.001, respectively) were also higher than in controls. E06-reactive oligodendrocytes (p=0.008) and the percentage of E06-reactive area (p=0.019) reached statistical significance only in the inactive lesions and not in the active lesions. Iron density decreased in inactive lesions compared with the surrounding NADGM tissue (p=0.019).

  • *The values represent median values and range. Significant p values after correction for multiple testing in comparison with controls.

  • †Significant p values after correction for multiple testing in comparison with NADGM.

  • APP, amyloid precursor protein.