Author | Study | # of patients positive versus screened | Primary or recurrent | Entire CMV genome present | Survival correlation |
---|---|---|---|---|---|
Cobbs et al23 | IHC on paraffin sections from 27 GBM surgical specimens obtained from non-immunocompromised patients. | 27/27 (100%) for IE1–72, 10/10 for mRNA, 6/6 for total genome | Not known | 6/6 analysed | Not known |
Mitchell et al50 | Detection of CMV proteins and nucleic acids in the peripheral blood and tumours of patients with GBM. | 42/45 for IE-1–72 IHC, 30/33 pp65 IHC, 16/16 ISH, 21/34 gB PCR, 8/34 IE1 PCR | 36 Primary | 16/16 analysed | Not known |
Scheurer et al51 | Immunohistochemical staining and in situ hybridisation specific for CMV antigen and DNA using highly optimised techniques with a set of well-characterised glioma tissue specimens. | 44/50 IHC for IE1 | Primary | Not present | Not known |
Bhattacharjee et al30 | Examined the CMV prevalence in malignant gliomas using different methods like immunoblotting, deep sequencing and genetic analysis across tumour tissue. | 16/17 by PCR | Not known | Not present | Not known |
Dos Santos et al31 | Tumour tissues and peripheral blood of patients with GBM were examined for the presence of CMV DNA. | 21/22 by qPCR and 20/22 by hemi-nested PCR | Primary | Not known | Not known |
Fonseca et al33 | Screened 75 Brazilian glioma biopsies for the presence of CMV DNA sequences. | 27/75 (36%) pp65 PCR | Primary | Not present | The survival of CMV DNA-positive patients was similar to CMV DNA-negative patients |
Lucas et al34 | IHC analysis of paraffin embedded tissue sections on 49newly diagnosed GBM tumours. | 25/49 pp65 IHC, 8/49 IE1 IHC | Primary | Not present | Not known |
Sabatier et al97 | Detect CMV infection in a series of French cases of CNS tumours by combining IHC and ISH on tissue microarrays. | 9 (confirmed)/132 by IHC, 7/132 by ISH | Not known | Not present | Not known |
Lau et al98 | Presence of CMV was evaluated by determining the expression of CMV proteins by IHC and the presence of viral genomes by ISH and PCR. | 0/22 | Not known | Not present | Not known |
Yamashita et al29 | Measured the number of viral DNA molecules per cell in both frozen and paraffin-embedded tumour biopsies using real-time quantitative PCR (QPCR). | 0/59 by qPCR | Not known | Not present | Not known |
Tang et al24 | Screened glioma samples for CMV based on more than 700 billion transcriptome sequencing reads from The Cancer Genome Atlas Research Network. | 0/167 by RNA-seq | Not known | Not present | Not known |
CMV, cytomegalovirus; GBM, glioblastoma multiforme; IHC, immunohistochemical staining; ISH, in situ hybridisation; PCR, polymerase chain reaction.