Assay metrics based on 66 AQP4 positive samples and 127 controls
Sensitivity | Specificity | LRs | Accuracy | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Assay number | Seropositive NMO (32) | Seropositive NMOSD (34) | TP total (66) | Sensitivity | 95% CI | Cons (92) | Seronegative NMO (18) | Seronegative NMOSD (17) | TN total (127) | Specificity | 95% CI | Positive LR | 95% CI | Negative LR | 95%CI | ((TP+TN)/193)×100 |
1 | 32 | 34 | 66 | 100.0 | 94.6 to 100 | 1 | 0 | 0 | 126 | 99.2 | 95.7 to 100 | 127 | 18.03 to 894.7 | 0 | 99.5 | |
2 | 31 | 34 | 65 | 98.5 | 91.8 to 100 | 1 | 0 | 2 | 124 | 97.6 | 93.3 to 99.5 | 41.7 | 13.6 to 127.6 | 0.02 | 0.00 to 0.11 | 97.9 |
4 | 32 | 30 | 62 | 93.9 | 85.2 to 98.3 | 0 | 0 | 0 | 127 | 100.0 | 97.1 to 100 | ∞ | 0.06 | 0.02 to 0.16 | 97.9 | |
5 | 31 | 30 | 61 | 92.4 | 83.2 to 97.5 | 0 | 0 | 0 | 127 | 100.0 | 97.1 to 100 | ∞ | 0.08 | 0.03 to 0.18 | 97.4 | |
6 | 31 | 30 | 61 | 92.4 | 83.2 to 97.5 | 0 | 0 | 0 | 127 | 100.0 | 97.1 to 100 | ∞ | 0.08 | 0.03 to 0.18 | 97.4 | |
8 | 31 | 30 | 61 | 92.4 | 83.2 to 97.5 | 0 | 0 | 0 | 127 | 100.0 | 97.1 to 100 | ∞ | 0.08 | 0.03 to 0.18 | 97.4 | |
7 | 32 | 29 | 61 | 92.4 | 83.2 to 97.5 | 1 | 0 | 0 | 126 | 99.2 | 95.7 to 100 | 117.4 | 16.6 to 827.9 | 0.08 | 0.03 to 0.18 | 96.9 |
18 | 31 | 34 | 65 | 98.5 | 91.8 to 100 | 4 | 0 | 1 | 122 | 96.1 | 91.1 to 98.7 | 25 | 10.6 to 59.1 | 0.02 | 0.00 to 0.11 | 96.9 |
14 | 32 | 34 | 66 | 100.0 | 94.6 to 100 | 5 | 2 | 0 | 120 | 94.5 | 89.0 to 97.8 | 18.1 | 8.8 to 37.3 | 0 | 96.4 | |
3 | 32 | 34 | 66 | 100.0 | 94.6 to 100 | 5 | 0 | 2 | 120 | 94.5 | 89.0 to 97.8 | 18.1 | 8.8 to 37.3 | 0 | 96.4 | |
9 | 31 | 30 | 61 | 92.4 | 83.2 to 97.5 | 2 | 0 | 0 | 125 | 98.4 | 94.4 to 99.8 | 58.7 | 14.8 to 232.5 | 0.08 | 0.03 to 0.18 | 96.4 |
10 | 28 | 29 | 57 | 86.4 | 75.7 to 93.6 | 0 | 0 | 0 | 127 | 100.0 | 97.1 to 100 | ∞ | 0.14 | 0.07 to 0.25 | 95.3 | |
11 | 29 | 27 | 56 | 84.8 | 73.9 to 92.5 | 0 | 0 | 0 | 127 | 100.0 | 97.1 to 100 | ∞ | 0.15 | 0.09 to 0.27 | 94.8 | |
19 | 27 | 29 | 56 | 84.8 | 73.9 to 92.5 | 0 | 0 | 0 | 127 | 100.0 | 97.1 to 100 | ∞ | 0.15 | 0.09 to 0.27 | 94.8 | |
16 | 29 | 26 | 55 | 83.3 | 72.1 to 91.4 | 0 | 0 | 0 | 127 | 100.0 | 97.1 to 100 | ∞ | 0.17 | 0.1 to 0.29 | 94.3 | |
15 | 32 | 32 | 64 | 97.0 | 89.5 to 99.6 | 5 | 2 | 4 | 116 | 91.3 | 85.0 to 95.6 | 11.2 | 6.4 to 19.7 | 0.03 | 0.01 to 0.13 | 93.3 |
13 | 27 | 26 | 53 | 80.3 | 68.7 to 89.1 | 0 | 0 | 1 | 126 | 99.2 | 95.7 to 99.98 | 102 | 14.4 to 721.1 | 0.2 | 0.12 to 0.32 | 92.7 |
21 | 28 | 27 | 55 | 83.3 | 72.1 to 91.4 | 3 | 0 | 1 | 123 | 96.9 | 92.1 to 99.1 | 26.5 | 10.0 to 69.8 | 0.17 | 0.1 to 0.3 | 92.2 |
12 | 31 | 31 | 62 | 93.9 | 85.2 to 98.3 | 9 | 4 | 5 | 109 | 85.8 | 78.5 to 91.4 | 6.6 | 4.3 to 10.2 | 0.07 | 0.03 to 0.18 | 88.6 |
17 | 24 | 22 | 46 | 69.7 | 57.2 to 80.4 | 9 | 2 | 1 | 115 | 90.6 | 84.1 to 95.0 | 7.4 | 4.2 to 12.9 | 0.33 | 0.23 to 0.48 | 83.4 |
20 | 17 | 17 | 34 | 51.5 | 38.9 to 64.0 | 2 | 2 | 0 | 123 | 96.9 | 92.1 to 99.1 | 16.4 | 6.1 to 44.1 | 0.5 | 0.39 to 0.64 | 81.3 |
The assays are ordered on the basis of accuracy, which is calculated as (((TP+TN)/total tests)×100). The sensitivity, specificity, positive and negative LRs, and their 95% CIs from 21 individual assays to detect AQP4-antibodies from 15 European diagnostic centres are presented along with assay accuracy.
Assays 5 and 6 use similar AQP4-M1 transfected cells that form part of two different biochip mosaics.
Cons, controls; LR, likelihood ratio; NMO, neuromyelitis optica; NMOSD, NMO spectrum disorder; TN, true negative; TP, true positive.