Table 3

Assay metrics based on 66 AQP4 positive samples and 127 controls

	Sensitivity					Specificity						LRs				Accuracy
Assay number	Seropositive NMO (32)	Seropositive NMOSD (34)	TP total (66)	Sensitivity	95% CI	Cons (92)	Seronegative NMO (18)	Seronegative NMOSD (17)	TN total (127)	Specificity	95% CI	Positive LR	95% CI	Negative LR	95%CI	((TP+TN)/193)×100
1	32	34	66	100.0	94.6 to 100	1	0	0	126	99.2	95.7 to 100	127	18.03 to 894.7	0		99.5
2	31	34	65	98.5	91.8 to 100	1	0	2	124	97.6	93.3 to 99.5	41.7	13.6 to 127.6	0.02	0.00 to 0.11	97.9
4	32	30	62	93.9	85.2 to 98.3	0	0	0	127	100.0	97.1 to 100	∞		0.06	0.02 to 0.16	97.9
5	31	30	61	92.4	83.2 to 97.5	0	0	0	127	100.0	97.1 to 100	∞		0.08	0.03 to 0.18	97.4
6	31	30	61	92.4	83.2 to 97.5	0	0	0	127	100.0	97.1 to 100	∞		0.08	0.03 to 0.18	97.4
8	31	30	61	92.4	83.2 to 97.5	0	0	0	127	100.0	97.1 to 100	∞		0.08	0.03 to 0.18	97.4
7	32	29	61	92.4	83.2 to 97.5	1	0	0	126	99.2	95.7 to 100	117.4	16.6 to 827.9	0.08	0.03 to 0.18	96.9
18	31	34	65	98.5	91.8 to 100	4	0	1	122	96.1	91.1 to 98.7	25	10.6 to 59.1	0.02	0.00 to 0.11	96.9
14	32	34	66	100.0	94.6 to 100	5	2	0	120	94.5	89.0 to 97.8	18.1	8.8 to 37.3	0		96.4
3	32	34	66	100.0	94.6 to 100	5	0	2	120	94.5	89.0 to 97.8	18.1	8.8 to 37.3	0		96.4
9	31	30	61	92.4	83.2 to 97.5	2	0	0	125	98.4	94.4 to 99.8	58.7	14.8 to 232.5	0.08	0.03 to 0.18	96.4
10	28	29	57	86.4	75.7 to 93.6	0	0	0	127	100.0	97.1 to 100	∞		0.14	0.07 to 0.25	95.3
11	29	27	56	84.8	73.9 to 92.5	0	0	0	127	100.0	97.1 to 100	∞		0.15	0.09 to 0.27	94.8
19	27	29	56	84.8	73.9 to 92.5	0	0	0	127	100.0	97.1 to 100	∞		0.15	0.09 to 0.27	94.8
16	29	26	55	83.3	72.1 to 91.4	0	0	0	127	100.0	97.1 to 100	∞		0.17	0.1 to 0.29	94.3
15	32	32	64	97.0	89.5 to 99.6	5	2	4	116	91.3	85.0 to 95.6	11.2	6.4 to 19.7	0.03	0.01 to 0.13	93.3
13	27	26	53	80.3	68.7 to 89.1	0	0	1	126	99.2	95.7 to 99.98	102	14.4 to 721.1	0.2	0.12 to 0.32	92.7
21	28	27	55	83.3	72.1 to 91.4	3	0	1	123	96.9	92.1 to 99.1	26.5	10.0 to 69.8	0.17	0.1 to 0.3	92.2
12	31	31	62	93.9	85.2 to 98.3	9	4	5	109	85.8	78.5 to 91.4	6.6	4.3 to 10.2	0.07	0.03 to 0.18	88.6
17	24	22	46	69.7	57.2 to 80.4	9	2	1	115	90.6	84.1 to 95.0	7.4	4.2 to 12.9	0.33	0.23 to 0.48	83.4
20	17	17	34	51.5	38.9 to 64.0	2	2	0	123	96.9	92.1 to 99.1	16.4	6.1 to 44.1	0.5	0.39 to 0.64	81.3

The assays are ordered on the basis of accuracy, which is calculated as (((TP+TN)/total tests)×100). The sensitivity, specificity, positive and negative LRs, and their 95% CIs from 21 individual assays to detect AQP4-antibodies from 15 European diagnostic centres are presented along with assay accuracy.
Assays 5 and 6 use similar AQP4-M1 transfected cells that form part of two different biochip mosaics.
Cons, controls; LR, likelihood ratio; NMO, neuromyelitis optica; NMOSD, NMO spectrum disorder; TN, true negative; TP, true positive.