Table 3

Assay metrics based on 66 AQP4 positive samples and 127 controls

SensitivitySpecificityLRsAccuracy
Assay numberSeropositive NMO (32)Seropositive NMOSD (34)TP total (66)Sensitivity95% CICons (92)Seronegative NMO (18)Seronegative NMOSD (17)TN total (127)Specificity95% CIPositive LR95% CINegative LR95%CI((TP+TN)/193)×100
1323466100.094.6 to 10010012699.295.7 to 10012718.03 to 894.70 99.5
231346598.591.8 to 10010212497.693.3 to 99.541.713.6 to 127.60.020.00 to 0.1197.9
432306293.985.2 to 98.3000127100.097.1 to 1000.060.02 to 0.1697.9
531306192.483.2 to 97.5000127100.097.1 to 1000.080.03 to 0.1897.4
631306192.483.2 to 97.5000127100.097.1 to 1000.080.03 to 0.1897.4
831306192.483.2 to 97.5000127100.097.1 to 1000.080.03 to 0.1897.4
732296192.483.2 to 97.510012699.295.7 to 100117.416.6 to 827.90.080.03 to 0.1896.9
1831346598.591.8 to 10040112296.191.1 to 98.72510.6 to 59.10.020.00 to 0.1196.9
14323466100.094.6 to 10052012094.589.0 to 97.818.18.8 to 37.30 96.4
3323466100.094.6 to 10050212094.589.0 to 97.818.18.8 to 37.30 96.4
931306192.483.2 to 97.520012598.494.4 to 99.858.714.8 to 232.50.080.03 to 0.1896.4
1028295786.475.7 to 93.6000127100.097.1 to 1000.140.07 to 0.2595.3
1129275684.873.9 to 92.5000127100.097.1 to 1000.150.09 to 0.2794.8
1927295684.873.9 to 92.5000127100.097.1 to 1000.150.09 to 0.2794.8
1629265583.372.1 to 91.4000127100.097.1 to 1000.170.1 to 0.2994.3
1532326497.089.5 to 99.652411691.385.0 to 95.611.26.4 to 19.70.030.01 to 0.1393.3
1327265380.368.7 to 89.100112699.295.7 to 99.9810214.4 to 721.10.20.12 to 0.3292.7
2128275583.372.1 to 91.430112396.992.1 to 99.126.510.0 to 69.80.170.1 to 0.392.2
1231316293.985.2 to 98.394510985.878.5 to 91.46.64.3 to 10.20.070.03 to 0.1888.6
1724224669.757.2 to 80.492111590.684.1 to 95.07.44.2 to 12.90.330.23 to 0.4883.4
2017173451.538.9 to 64.022012396.992.1 to 99.116.46.1 to 44.10.50.39 to 0.6481.3
  • The assays are ordered on the basis of accuracy, which is calculated as (((TP+TN)/total tests)×100). The sensitivity, specificity, positive and negative LRs, and their 95% CIs from 21 individual assays to detect AQP4-antibodies from 15 European diagnostic centres are presented along with assay accuracy.

  • Assays 5 and 6 use similar AQP4-M1 transfected cells that form part of two different biochip mosaics.

  • Cons, controls; LR, likelihood ratio; NMO, neuromyelitis optica; NMOSD, NMO spectrum disorder; TN, true negative; TP, true positive.