Assays
| Assay number | Assay | Isoform | Transfection | [Serum] | Detection | Quantitative |
|---|---|---|---|---|---|---|
| 1 | Live CBA | M23 | Transient | 1:20 | Fluorescence | Semi |
| 2 | Live CBA | M23 | Transient | 1:20 | Fluorescence | Semi |
| 3 | Live CBA | M23 | Transient | 1:20 | Fluorescence | Semi |
| 4 | Fixed CBA EI | M23 | Transient | 1:10 | Fluorescence | Semi |
| 5 | Fixed CBA EI | M1 | Transient | 1:10 | Fluorescence | Semi |
| 6 | Fixed CBA EI | M1 | Transient | 1:10 | Fluorescence | Semi |
| 7 | Fixed CBA EO | M1 | Transient | 1:10 | Fluorescence | Semi |
| 8 | Fixed CBA EO | M1 | Transient | 1:10 | Fluorescence | Semi |
| 9 | Fixed CBA EO | M1 | Transient | 1:10 | Fluorescence | Semi |
| 10 | Fixed CBA EO | M1 | Transient | 1:10 | Fluorescence | Semi |
| 11 | Fixed CBA EO | M1 | Transient | 1:60 | Fluorescence | Semi |
| 12 | Fixed CBA EO | M1 | Transient | 1:10 | Fluorescence | Semi |
| 13 | Fixed CBA EO | M1 | Transient | 1:10 | Fluorescence | Semi |
| 14 | FACS | M23 | Transient | 1:20 | Fluorescence | Yes |
| 15 | FACS | M23 | Stable | 1:100 | Fluorescence | Yes |
| 16 | FACS | M23 | Transient | 1:50 | Fluorescence | Yes |
| 17 | FACS | M1 | Stable | 1:100 | Fluorescence | Yes |
| 18 | TBA-IHC-rat | NA | NA | 1:200 | Colorimetric | Semi |
| 19 | TBA-IIF-rat/monkey | NA | NA | 1:10 | Fluorescence | Semi |
| 20 | TBA-IIF-mouse | NA | NA | 1:60 | Fluorescence | Semi |
| 21 | ELISA | M23 | NA | 2:3 | Colorimetric | Yes |
The basic characteristics of each assay are presented in six columns.
FACS, flow cytometry; Fixed CBA EI, fixed cell-based assay done in-house at Euroimmun AG; Fixed CBA EO, fixed cell-based assay (Euroimmun AG commercial assay) run at different European diagnostic centres; Live CBA, live cell-based assay; NA, not applicable; TBA-IHC, indirect immunohistochemistry on tissue sections; TBA-IIF, indirect immunofluorescence on tissue sections.