Summary of cytokine and chemokine levels secreted by PnMECs after exposure to a patient’s sera
| HC (n=12) | ALS (n=9) | t-CIDP (n=12) | MADSAM (n=10) | MMN (n=11) | P values (Bonferroni correction) | |
| Mean (SD) | Mean (SD) | Mean (SD) | Mean (SD) | Mean (SD) | ||
| IL-7 | 10.9 (1.2) | 10.7 (1.2) | 10.0 (1.8) | 16.2 (2.9) | 13.2 (5.4) | <0.001 (MADSAM vs t-CIDP) |
| VEGF | 119 (14) | 116 (15) | 103 (29) | 178 (41) | 133 (38) | <0.001 (MADSAM vs HC, t-CIDP, MMN) |
| G-CSF | 491 (91) | 511 (90) | 458 (106) | 778 (261) | 559 (171) | <0.001 (MADSAM vs ALS) |
| IP-10 | 44.2 (4.6) | 51.7 (6.5) | 48.7 (5.8) | 149 (34) | 92.1 (41.1) | <0.001 (MADSAM vs HC, ALS, t-CIDP, MMN) <0.001 (MMN vs HC, t-CIDP) |
| IL-4 | 0.41 (0.44) | 0.99 (0.41) | 1.12 (0.53) | 0.41 (1.28) | 1.27 (1.73) | NS |
| IL-9 | 3.88 (2.04) | 4.7 4 (1.39) | 3.17 (1.76) | 6.89 (2.80) | 5.72 (2.36) | NS |
| IL-12 | 0 (0) | 0.69 (2.09) | 2.42 (3.02) | 4.39 (3.60) | 4.45 (3.93) | NS |
| PDGF-BB | 86.4 (20.0) | 82.4 (38.5) | 133 (43) | 150 (71) | 168 (108) | NS |
| TNF-α | 20.7 (71.8) | 87.5 (118) | 133 (125) | 68.1 (144) | 130 (268) | NS |
| IL-1β | 0 | 0 | 0 | 0.32 (0.66) | 0.29 (0.66) | NS |
| IL-5 | 0.43 (0.63) | 1.07 (0.62) | 0.78 (0.70) | 1.15 (0.90) | 1.05 (1.17) | NS |
| IL-6 | 0 (0) | 0.17 (0.52) | 0.83 (1.13) | 0.46 (0.75) | 0.79 (1.43) | NS |
| IL-8 | 4.85 (1.24) | 6.70 (1.56) | 6.43 (2.15) | 5.98 (3.20) | 7.43 (5.17) | NS |
| IL-13 | 1.88 (0.21) | 2.15 (0.30) | 2.15 (0.37) | 2.34 (0.48) | 2.18 (0.73) | NS |
| IL-1ra | 0 (0) | 1.89 (5.68) | 3.73 (8.87) | 12.8 (40.4) | 10.7 (20.7) | NS |
| FGF-2 | 243 (56) | 253 (70) | 256 (39) | 212 (44) | 300 (233) | NS |
| Eotaxin | 3.65 (1.73) | 5.23 (1.87) | 3.96 (1.85) | 7.06 (4.49) | 4.35 (3.33) | NS |
| RANTES | 272 (82) | 249 (93) | 228 (136) | 207 (121) | 249 (110) | NS |
| MIP-1a | 0 (0) | 0.06 (0.18) | 0.06 (0.14) | 0.30 (0.95) | 0.22 (0.56) | NS |
Undetermined cytokines: IL-2, IL-10, IL-15, IL-17, GM-CSF, MCP-1, MIP-1b, IFN-γ.
ALS, amyotrophic lateral sclerosis; FGF, fibroblast growth factor; G-CSF, granulocyte colony-stimulating factor; GM-CSF, granulocyte-macrophage colony-stimulating factor; HC, healthy controls; IFN-γ, interferon-γ; IL, interleukin; IL-1ra, IL-1 receptor antagonist; IP-10, inducible protein of 10 kDa; MADSAM, multifocal acquired demyelinating sensory and motor neuropathy; MCP-1, macrophage chemoattractant protein-1; MIP, macrophage inflammatory protein; MMN, multifocal motor neuropathy; PDGF, platelet-derived growth factor; PnMECS, peripheral nerve microvascular endothelial cells; RANTES, CCL4/MIP-1β andCCL5/regulated on activation, normal T cell expressed and secreted; t-CIDP, typical chronic inflammatory demyelinating polyneuropathy; TNF-α, tumour necrosis factor-α; VEGF, vascular endothelial growth factor.