Time-Dependent Impairment of Inhibitory Avoidance Retention in Rats by Posttraining Infusion of a Mitogen-Activated Protein Kinase Kinase Inhibitor into Cortical and Limbic Structures

doi:10.1006/nlme.1999.3913

Neurobiology of Learning and Memory

Volume 73, Issue 1, January 2000, Pages 11-20

https://doi.org/10.1006/nlme.1999.3913 Get rights and content

Abstract

Mitogen-activated protein kinase (MAPK) is abundantly expressed in postmitotic neurons of the developed nervous system. MAPK is activated and required for induction of long-term potentiation (LTP) in the CA1 area of the hippocampus, which is blocked by the specific inhibitor of the MAPK kinase, PD 098059. Recently it was demonstrated that MAPK is activated in the hippocampus after training and is necessary for contextual fear conditioning learning. The present work tests the role of the MAPK cascade in step-down inhibitory avoidance (IA) retention. PD 098059 (50 μM) was bilaterally injected (0.5 μl/side) into the CA1 region of the dorsal hippocampus or entorhinal cortex at 0, 90, 180, or 360 min, or into the amygdala or parietal cortex at 0, 180, or 360 min after IA training in rats using a 0.4-mA foot shock. Retention testing was carried out 24 h after training. PD 098059 impaired retention when injected into the dorsal hippocampus at 180 min, but not 0, 90, and 360 min after training. When infused into the entorhinal cortex, PD 098059 was amnestic at 0 and 180 min, but not at 90 and 360 min after training. The MAPKK inhibitor also impairs IA retention when infused into the parietal cortex immediately after training, but not at 180 or 360 min. Infusions performed into amygdala were amnestic at 180 min, but not at 0 and 360 min after training. Our results suggest a time-dependent involvement of the MAPK cascade in the posttraining memory processing of IA; the time dependency is different in the hippocampus, amygdala, entorhinal cortex, or parietal cortex of rats.

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^☆: This work was supported by PRONEX and Fundaçá VITAE, Brazil.

^☆☆: Address correspondence and reprint request to Roger Walz, Centro de Memória, Departamento de Bioquı́mica, Instituto de Ciências Básicas da Saúde, UFRGS, Ramiro Barcelos 2600 (Anexo), 90035-003 Porto Alegre, RS, Brazil. Fax: 55 516 5535. E-mail: [email protected].

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Neurobiology of Learning and Memory

Abstract

References (27)

PD098059 is a specific inhibitor of the activation of mitogen-activated protein kinase kinase in vitro and in vivo

Journal of Biological Chemistry

Late and prolonged post-training memory modulation in entorhinal and parietal cortex by drugs acting on the cAMP/protein kinase A signalling pathway

Behavioural Pharmacology

The MAPK cascade is required for mammalian associative learning

Nature Neuroscience

Mutation in the phosphorylation sites of MAP kinase blocks learning-related internalization of apCAM in Aplysia sensory neurons

Neuron

Potassium chloride pulse enhances mitogen-activated protein kinase activity in rat hippocampal slices

Journal of Neurochemistry

Involvement of hippocampal cAMP/cAMP-dependent protein kinase signaling pathways in a late memory consolidation phase of aversively motivated learning in rats

Proceedings of the National Academy of Sciences USA

Drugs acting upon the cyclic adenosine monophosphate/protein kinase A signalling pathway modulate memory consolidation when given after training into rat hippocampus but not amygdala

Behavioural Pharmacology

A role for the Ras signalling pathway in synaptic transmission and long-term memory

Nature

Activation of p42 mitogen-activated protein kinase in hippocampal long term potentiation

Journal of Biological Chemistry

A requirement for the mitogen-activated protein kinase cascade in hippocampal long term potentiation

Journal of Biological Chemistry

Ca⁺⁺-dependent routs to Ras: Mechanisms for neuronal survival, differentiation, and plasticity

Cell

The use of avoidance training in studies of modulation of memory storage

Behavioral and Neural Biology

Protein kinases and phosphatase: The yin and yang of protein phosphoriylation and signaling

Cell

Cited by (92)

Transplantation of ERK gene-modified bone marrow mesenchymal stem cells ameliorates cognitive deficits in a 6-hydroxydopamine rat model of Parkinson's disease

The Entorhinal Cortex as a Gateway for Amygdala Influences on Memory Consolidation

What animal models can tell us about long-term cognitive dysfunction following sepsis: A systematic review

Effects of sub-chronic caffeine ingestion on memory and the hippocampal Akt, GSK-3β and ERK signaling in mice

The effect of cinnamaldehyde on passive avoidance memory and hippocampal Akt, ERK and GSK-3β in mice

Potential of GPCRs to modulate MAPK and mTOR pathways in Alzheimer's disease

Regular ArticleTime-Dependent Impairment of Inhibitory Avoidance Retention in Rats by Posttraining Infusion of a Mitogen-Activated Protein Kinase Kinase Inhibitor into Cortical and Limbic Structures☆,☆☆

Abstract

PD098059 is a specific inhibitor of the activation of mitogen-activated protein kinase kinase in vitro and in vivo

Journal of Biological Chemistry

Late and prolonged post-training memory modulation in entorhinal and parietal cortex by drugs acting on the cAMP/protein kinase A signalling pathway

Behavioural Pharmacology

The MAPK cascade is required for mammalian associative learning

Nature Neuroscience

Mutation in the phosphorylation sites of MAP kinase blocks learning-related internalization of apCAM in Aplysia sensory neurons

Neuron

Potassium chloride pulse enhances mitogen-activated protein kinase activity in rat hippocampal slices

Journal of Neurochemistry

Involvement of hippocampal cAMP/cAMP-dependent protein kinase signaling pathways in a late memory consolidation phase of aversively motivated learning in rats

Proceedings of the National Academy of Sciences USA

Drugs acting upon the cyclic adenosine monophosphate/protein kinase A signalling pathway modulate memory consolidation when given after training into rat hippocampus but not amygdala

Behavioural Pharmacology

A role for the Ras signalling pathway in synaptic transmission and long-term memory

Nature

Activation of p42 mitogen-activated protein kinase in hippocampal long term potentiation

Journal of Biological Chemistry

A requirement for the mitogen-activated protein kinase cascade in hippocampal long term potentiation

Journal of Biological Chemistry

Ca++-dependent routs to Ras: Mechanisms for neuronal survival, differentiation, and plasticity

Cell

The use of avoidance training in studies of modulation of memory storage

Behavioral and Neural Biology

Protein kinases and phosphatase: The yin and yang of protein phosphoriylation and signaling

Cell

Regular Article
Time-Dependent Impairment of Inhibitory Avoidance Retention in Rats by Posttraining Infusion of a Mitogen-Activated Protein Kinase Kinase Inhibitor into Cortical and Limbic Structures☆,☆☆

Ca⁺⁺-dependent routs to Ras: Mechanisms for neuronal survival, differentiation, and plasticity