Summary
Brain microdialysis was used to characterize extracellular gamma-aminobutyric acid (GABA) in the substantia nigra reticulata (SNR) of freely moving rats. The extracellular GABA in the SNR was characterized using acutely implanted probes (4–8 h after surgery; day 1) and chronically implanted probes (24 h after surgery; day 2).
3-Mercaptopropionic acid, a glutamic acid decarboxylase inhibitor, was used to identify GABA. This drug induced an immediate decrease in the extracellular GABA levels to 40% of basal values, suggesting that the detected GABA is, at least in part, newly synthesized.
The basal levels of extracellular GABA measured either on day 1 or day 2 were not affected by infusion of micromolar amounts of tetrodotoxin. Therefore, a direct coupling between GABA dialysate concentrations and nerve-impulse flow does not seem to exist. Infusion of the GABA uptake inhibitor nipecotic acid (0.5 mmol/l) resulted in a 4-fold increase in the dialysate levels of GABA lasting at least for 3 h on both days. K+ stimulation (60 mmol/l) increased extracellular GABA levels in the SNR to 450% of basal values. This effect again did not differ significantly on day 1 and day 2.
The origin of the extracellular GABA in the SNR, as recorded by microdialysis under the two experimental conditions, is discussed.
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Timmerman, W., Zwaveling, J. & Westerink, B.H.C. Characterization of extracellular GABA in the substantia nigra reticulata by means of brain microdialysis. Naunyn-Schmiedeberg's Arch Pharmacol 345, 661–665 (1992). https://doi.org/10.1007/BF00164580
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DOI: https://doi.org/10.1007/BF00164580