Summary
Immunoglobulin G (IgG) was isolated from three multiple sclerosis (MS) and two control brains by Protein A Sepharose affinity chromatography and was characterized by thin-layer polyacrylamide gel isoelectric focusing (PAG IEF) and antiserum immunofixation. The three MS brains contained oligoclonal IgG. Immunofixation with measles, herpes simplex, varicella, rubella, mumps and cytomegalovirus as antigens and autoradiography revealed that some of the oligoclonal IgG bands separated by PAG IEF contained antibodies against herpes simplex in one, measles in two and varicella virus in all three MS brains. No antibodies were detected with this technique against structural human (crude saline, lipid-proteolipid, ganglioside, and myelin basic protein extracts of MS and normal human brain) and bovine (purified myelin, myelin basic protein and oligodendrocytes of bovine brain) brain components. The finding of viral antibodies and the absence of antibodies against structural brain proteins in oligoclonal MS brain IgG is similar to that previously recorded in MS cerebrospinal fluid (CSF).
Zusammenfassung
Immunoglobulin G, IgG, wurde aus der Gehirnsubstanz von drei Fällen mit multipler Sklerose (MS) und zwei Kontrollen mittels Affinitätschromatographie mit Protein A-Sepharose isoliert. Das IgG wurde mit isoelektrischer Fokussierung in Polyacrylamidgelplatten (PAG IEF) und anschließender Antiserum-Immunofixation charakterisiert. Bei MS wurde oligoklonales IgG gefunden. Mit Hilfe von Antigen-Immunofixation mit Masern, Herpes simplex, Varizellen, Rubella, Parotitis und Zytomegalievirus und nachfolgender Autoradiographie (radioaktive Zweitantikörper gegen die genannten Antigene) konnte gezeigt werden, daß die oligoklonalen Bande in den drei MS-Gehirnen Antikörper gegen Herpes simplex in einem, Masern in zwei und Varizellen-Virus in allen drei Fällen enthielten. Keine solchen Antikörper wurden entdeckt, wenn die Technik für strukturelle Gehirn-Komponenten vom Menschen (einfache physiologische Salz-, Lipid-Proteolipid-, Gangliosid- und basische Myelo-Protein-Extrakte von MS und normalem Gehirn) und vom Rind (gereinigtes Myelin, basisches Myelo-Protein und Oligo-Dendrozyten aus Gehirnrinde) verwendet wurde.
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Roström, B. Antibodies against viruses and structural brain components in oligoclonal IgG obtained from multiple sclerosis brain. J Neurol 226, 255–263 (1982). https://doi.org/10.1007/BF00313398
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DOI: https://doi.org/10.1007/BF00313398