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Cutaneous innervation in man visualized with protein gene product 9.5 (PGP 9.5) antibodies

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Summary

Using antibodies to the neuronal cytoplasmic protein, protein gene product 9.5 (PGP 9.5) the cutaneous innervation in man was investigated. The distribution of PGP 9.5 immunoreactive nerve fibers was compared with the distribution of nerve fibers immunoreactive to neuron specific enolase, neurofilament proteins, calcitonin gene related peptide, vasoactive intestinal polypeptide and neuropeptide Y. PGP 9.5 immunoreactive nerve fibers were found in the epidermis, dermis, in Meissner's corpuscles, innervating Merkel cells, around blood vessels, sweat glands and hair follicles. Merkel cells were also PGP 9.5 positive. The labelled nerve fibers included sensory and autonomic fibers, visualizing the whole innervation of the human skin. The number of positive fibers and the intensity of the fluorescence was greater with PGP 9.5 antibodies than with any of the other markers included. Thus, PGP 9.5 antibodies may serve as a tool for investigations of cutaneous innervation, reinnervation and nerve regeneration in different clinical conditions.

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References

  • Björklund H, Dalsgaard C-J, Jonsson C-E, Hermansson A (1986) Sensory and autonomic innervation of non-hairy and hairy human skin. An immunohistochemical study. Cell Tissue Res 243:51–57

    Google Scholar 

  • Coons AH (1958) Fluorescent antibody methods. In: Danielli JF (ed) General cytochemical methods. Academic Press, New York, pp 399–422

    Google Scholar 

  • Dalsgaard C-J, Jonsson C-E, Hökfelt T, Cuello AC (1983) Localization of substance P immunoreactive nerve fibers in human digital skin. Experientia 39:1018–1020

    Google Scholar 

  • Dalsgaard C-J, Björklund H, Jonsson C-E, Hermansson A, Dahl D (1984) Distribution of neurofilament-immunoreactive nerve fibers in human digital skin. Histochemistry 81:111–114

    Google Scholar 

  • Dalsgaard C-J, Jernbeck J, Stains W, Kjartansson J, Hægerstrand A, Hökfelt T, Brodin E, Cuello AC, Brown JC (1989) Calcitonin gene-related peptide like immunoreactivity in nerve fibers in the human skin: Relation to fibers containing substance P-, somatostatin- and vasoactive intestinal polypeptide-like immunoreactivity. Histochemistry 91:35–38

    Google Scholar 

  • Gibbins IL, Wattchow D, Coventry B (1987) Two immunohistochemically identified populations of calcitonin gene-related peptide (CGRP)-immunoreactive axons in human skin. Brain Res 414:143–148

    Google Scholar 

  • Gulbenkain S, Wharton J, Polak JM (1987) The visualization of cardiovascular innervation in the guinea-pig using an antiserum to protein gene product 9.5 (PGP 9.5). J Auton Nerv Syst 19:581–593

    Google Scholar 

  • Hermansson A, Dalsgaard C-J (1987) Sensory reinnervation and sensibility in skin transplants. Med Biol 65:49–52

    Google Scholar 

  • Hermansson A, Dalsgaard C-J, Arnander C, Lindblom U (1987a) Sensibility and reinnervation in free flaps. Plast Reconstr Surg 79:422–425

    Google Scholar 

  • Hermansson A, Dalsgaard C-J, Jonsson C-E, Björklund H, Dahl D (1987b) A study of the cutaneous sensory reinnervation after burn injury using antibodies to neurofilament. Eur J Plast Reconstr Surg 142:355–358

    Google Scholar 

  • Hermansson A, Dalsgaard C-J, Lindblom U, Björklund H (1987c) Sensory reinnervation and sensibility after superficial skin wounds in human patients. Neurosci Lett 74:377–382

    Google Scholar 

  • Jackson GD, Thompson RJ (1981) The demonstration of new human brain-specific proteins by high-resolution two-dimensional polyacrylamide gel electrophoresis. J Neurol Sci 49:429–438

    Google Scholar 

  • Johansson O, Vaalasti A (1987) Immunohistochemical evidence for the presence of somatostatin-containing sensory nerve fibers in the human skin. Neurosci Lett 73:225–230

    Google Scholar 

  • Lawson SN, Harper AA, Harper EI, Garson JA, Anderton BH (1984) A monoclonal antibody against neurofilament protein specifically labels a subpopulation of rat sensory neurones. J Comp Neurol 228:263–272

    Google Scholar 

  • Lundberg L-M, Alm P, Wharton J, Polak JM (1988) Protein gene product 9.5 (PGP 9.5). A new neuronal marker visualizing the whole uterine innervation and pregnancy-induced and developmental changes in the guinea pig. Histochemistry 90:9–17

    Google Scholar 

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Authors and Affiliations

  1. Department of Anatomy, Karolinska Institutet, Box 60400, S-10401, Stockholm, Sweden

    C.-J. Dalsgaard, M. Rydh & A. Hægerstrand

  2. Department of Plastic Surgery, Karolinska Hospital, Box 60500, S-10401, Stockholm, Sweden

    C.-J. Dalsgaard

Authors
  1. C.-J. Dalsgaard
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  2. M. Rydh
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  3. A. Hægerstrand
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Dalsgaard, CJ., Rydh, M. & Hægerstrand, A. Cutaneous innervation in man visualized with protein gene product 9.5 (PGP 9.5) antibodies. Histochemistry 92, 385–390 (1989). https://doi.org/10.1007/BF00492495

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  • DOI: https://doi.org/10.1007/BF00492495

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