Summary
A technique is described for isolating peripheral nerve fibres by teasing and subsequently examining them by light and electron microscopy. The technique was applied to the study of unusual swollen fibres observed central to the lesion in transected nerves in rats. These were shown to be the result of the demyelination of already remyelinated segments of the fibre.
Zusammenfassung
Es wird eine Technik zur Isolierung peripherer Nervenfasern durch Auffasern und nachfolgende licht-undd elektronenmikroskopische Untersuchung beschrieben. Diese Technik wurde zum Studium ungewöhnlich geschwollener Fasern angewandt, die proximal der Läsion bei durchschnittenen Nerven von Ratten beobachtet wurden. Diese Fasern wurden als das Ergebnis der Demyelinisation bereits remyelinisierter Segmente dargestellt.
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Spencer, P.S., Thomas, P.K. The examination of isolated nerve fibres by light and electron microscopy, with observations on demyelination proximal to neuromas. Acta Neuropathol 16, 177–186 (1970). https://doi.org/10.1007/BF00687357
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DOI: https://doi.org/10.1007/BF00687357