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Regulation of NaK-ATPase by platelet-derived growth factors in cultured rat thoracic aortic smooth muscle cells

  • Original Paper
  • Published:
Journal of Biomedical Science

Abstract

Regulation of (Na+ + K+)-adenosine triphosphatase (NaK-ATPase) by platelet-derived growth factor (PDGF) in cultured rat thoracic aortic smooth muscle cells (SMC) was examined. PDGF-BB enhances SMC proliferation and NaK-ATPase activity. Ouabain, an inhibitor of NaK-ATPase activity, prevents PDGF-BB-induced SMC proliferation. As shown by Western blot and immunochemiluminescence analysis, PDGF-BB also enhances α1, truncated α1, and β1 NaK-ATPase subunit levels. PDGF-AA and PDGF-AB show no effect on α1 and truncated α1 levels in slot blot analysis. Induction of NaK-ATPase subunit levels by PDGF-BB could be one of the initial events in vascular SMC proliferation.

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  1. Department of Physiology, Uniformed Services University of the Health Sciences, 4301 Jones Bridge Road, 20814, Bethesda, MD, USA

    Chu-Shek Lo PhD

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  1. Chu-Shek Lo PhD
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Lo, CS. Regulation of NaK-ATPase by platelet-derived growth factors in cultured rat thoracic aortic smooth muscle cells. J Biomed Sci 3, 92–97 (1996). https://doi.org/10.1007/BF02255536

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  • DOI: https://doi.org/10.1007/BF02255536

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