Blood vitamin B1, transketolase and thiamine pyrophosphate (TPP) effect in beriberi patients, with studies employing discriminant analysis

doi:10.1016/0009-8981(80)90001-7

Clinica Chimica Acta

Volume 108, Issue 2, 8 December 1980, Pages 159-168

https://doi.org/10.1016/0009-8981(80)90001-7 Get rights and content

Abstract

In recent years, many cases of beriberi have been reported throughout Japan. One may assume that a great many healthy subjects suffer from a subclinical thiamine deficiency. The present study was carried out in order to examine thiamine status in 21 beriberi patients and 674 apparently normal subjects. In the beriberi patients the total vitamin b₁ in whole blood and transketolase activity in the hemolysate were significantly lower, and the thiamine pyrophosphate effect was significantly higher, compared to normal subjects. However, these two groups could not be clearly separated by these biochemical parameters because of significant overlap. On analyzing the results of these biochemical parameters by discriminant analysis, beriberi could be diagnosed with an accuracy of 87,7%. Thus, vitamin b₁ levels in blood, transketolase activity and the thiamine pyrophosphate effect in the hemolysate are useful biochemical indices for the diagnosis of beriberi. Above all, the thiamine pyrophosphate effect proved to be the most effective parameter in distinguishing the beriberi group from normal subjects.

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Blood vitamin B1, transketolase and thiamine pyrophosphate (TPP) effect in beriberi patients, with studies employing discriminant analysis

Abstract

J. Nutr.

Brain Res.

Clin. Chim. Acta

Am. J. Clin. Nutr.

Am. J. Clin. Nutr.

Determination of thiamine by the thiochrom reaction; application of cyanogen bromide in place of potassium ferricyanide

Anal. Chem.

The pathogenesis of the peculiar polyneuritis with edema of lower extremities

Neurol. Med. (Tokyo)

The pathogenesis of the acute polyneuritis with cardiac enlargement and edema

Neurol. Med. (Tokyo)

Blood vitamin B₁, transketolase and thiamine pyrophosphate (TPP) effect in beriberi patients, with studies employing discriminant analysis