Elsevier

Neuroscience Letters

Volume 199, Issue 2, 20 October 1995, Pages 152-154
Neuroscience Letters

Induction of nitrotyrosine-like immunoreactivity in the lower motor neuron of amyotrophic lateral sclerosis

https://doi.org/10.1016/0304-3940(95)12039-7Get rights and content

Abstract

Spinal cords of sporadic cases with amyotrophic lateral sclerosis (ALS) and normal controls were immunohistochemically examined using antibodies for Cu/Zn superoxide dismutase (SOD) and nitrotyrosine (NT). Immunoreactivity for Cu/Zn SOD of the motor neurons was not different between the ALS and controls. In contrast, immunoreactivity for NT was densely detected in motor neurons of ALS but was not or was only minimally detected in those of controls. The staining was also found in the axons of motor neurons of ALS, but was not found in the controls. These results suggest that nitration of protein-tyrosine residue is upregulated in motor neurons of the spinal cord of ALS.

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    This work was partly supported by Grant-in-Aid for Scientific Research on Priority Areas (Kanazawa I) 06272204,and 07264204, and Grant-in-Aid for Scientific Research (B) 07457152 and (C) 06807055 from the Ministry of Education, Science and Culture of Japan, and by a grant (Hirai S) from the Ministry of Health and Welfare of Japan.

    The authors appreciate cooperation of the patients and their families with this project. We thank J.S. Beckman, T. Siddique, and RH. Brown Jr. for the generous discussion and support.

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