Elsevier

Neuroscience Letters

Volume 230, Issue 2, 18 July 1997, Pages 117-120
Neuroscience Letters

Plasma extravasation and neuropeptide release in human skin as measured by intradermal microdialysis

https://doi.org/10.1016/S0304-3940(97)00494-1Get rights and content

Abstract

Time course of plasma extravasation in neurogenic inflammation elicited by histamine iontophoresis and topical capsaicin application was measured in vivo by a modified intradermal microdialysis technique in humans. Single linear plasmapheresis fibers were inserted intracutaneously at the volar forearm and perfused with Ringer's solution (4 μl/min) with one microdialysis fiber located at the planned stimulation site and a second inside the axon reflex area. Protein concentration in the dialysate increased by 300% in the histamine wheal but no increase was found in the surrounding flare area. There was no protein extravasation in the skin treated with capsaicin nor in the surrounding flare region. Inside the histamine-induced flare reaction a significant increase of calcitonin gene related peptide (CGRP) was found, whereas the increase of substance P (SP) was not significant.

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Acknowledgements

This work was supported by the Deutsche Forschungsgemeinschaft (SFB 353). We would like to acknowledge Professor Handwerker for his fruitful comments on the manuscript.

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