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Cloning, sequencing and expression of complementary DNA encoding the muscarinic acetylcholine receptor

Abstract

Cloning and sequence analysis of DNA complementary to porcine cerebral messenger RNA encoding the muscarinic acetylcholine receptor predict the complete amino-acid sequence of this protein. Expression of the complementary DNA produces functional muscarinic receptor in Xenopus oocytes. The muscarinic receptor is homologous with the β-adrenergic receptor and rhodopsin in both amino-acid sequence and suggested transmembrane topography.

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Kubo, T., Fukuda, K., Mikami, A. et al. Cloning, sequencing and expression of complementary DNA encoding the muscarinic acetylcholine receptor. Nature 323, 411–416 (1986). https://doi.org/10.1038/323411a0

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