Abstract
This study was designed to assess some of the contributions of excitatory amino acids to locally evoked responses in neurons in slices from frontal motor cortex in Sprague-Dawley rats. Intracellular recordings were obtained from 54 cortical neurons. Paroxysmal depolarization shifts (PDS) were evoked by local single pulse stimulation in cortex or in a small number of cases (n = 2) occurred spontaneously. These potentials could be abolished by application of kynurenic acid, a broad spectrum excitatory amino acid receptor antagonist. They were enhanced in Mg(2+)-free medium and could then be antagonized by application of D,L-2-amino-5-phosphonovalerate (AP5), a selective blocker of the N-methyl-D-aspartate (NMDA) receptors.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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2-Amino-5-phosphonovalerate / pharmacology
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Amino Acids*
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Animals
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Frontal Lobe / cytology
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Frontal Lobe / drug effects
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Frontal Lobe / physiology*
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In Vitro Techniques
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Kynurenic Acid
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Magnesium / physiology
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Membrane Potentials / drug effects
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Membrane Potentials / physiology
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Motor Cortex / cytology
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Motor Cortex / drug effects
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Motor Cortex / physiology*
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Neurons / drug effects
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Neurons / physiology*
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Rats
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Rats, Inbred Strains
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Receptors, Amino Acid
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Receptors, Cell Surface / physiology*
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Receptors, N-Methyl-D-Aspartate / antagonists & inhibitors
Substances
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Amino Acids
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Receptors, Amino Acid
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Receptors, Cell Surface
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Receptors, N-Methyl-D-Aspartate
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2-Amino-5-phosphonovalerate
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Kynurenic Acid
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Magnesium