Abstract
An isocratic high-performance liquid chromatographic (HPLC) method was developed for the simultaneous determination of lactate, pyruvate, and ascorbate in tissue and blood, sampled in vivo using the microdialysis technique. The compounds were separated on a silica-based, reversed-phase column or on a resin-based, cation exchange column. The acids were monitored by ultraviolet detection.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adipose Tissue / analysis*
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Animals
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Ascorbic Acid / analysis*
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Ascorbic Acid / blood
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Brain Chemistry*
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Chromatography, High Pressure Liquid
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Dialysis
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Lactates / analysis*
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Lactates / blood
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Muscles / analysis*
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Pyruvates / analysis*
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Pyruvates / blood
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Rats
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Spectrophotometry, Ultraviolet
Substances
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Lactates
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Pyruvates
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Ascorbic Acid