Abstract
Previous studies have shown that sphingolipids may be enriched in caveolae, plasmalemmal invaginations implicated in endocytosis and signal transduction. We synthesised a radiolabeled derivative of ganglioside GM1 bearing a photo-reactive cross-linker at the end of its acyl chain. After insertion in the plasma membrane of cultured A431 or MDCK cells and photoactivation, the main protein cross-linked by the GM1 derivative was VIP21-caveolin, an essential structural component of caveolae. This result shows close proximity between GM1 molecules and VIP21-caveolin in the caveolar membrane and strongly implicates sphingolipid segregation in the biogenesis of caveolae.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Autoradiography
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Carbohydrate Sequence
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Carrier Proteins / drug effects
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Carrier Proteins / isolation & purification
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Carrier Proteins / metabolism*
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Caveolin 1
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Caveolins*
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Cell Line
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Cell Membrane / metabolism
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Cross-Linking Reagents / pharmacology*
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Dogs
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Electrophoresis, Gel, Two-Dimensional
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G(M1) Ganglioside / analogs & derivatives*
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Humans
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Kidney
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Membrane Proteins / drug effects
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Membrane Proteins / isolation & purification
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Membrane Proteins / metabolism*
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Molecular Sequence Data
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Tritium
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Tumor Cells, Cultured
Substances
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CAV1 protein, human
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Carrier Proteins
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Caveolin 1
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Caveolins
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Cross-Linking Reagents
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Membrane Proteins
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Tritium
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G(M1) Ganglioside