A photo-reactive derivative of ganglioside GM1 specifically cross-links VIP21-caveolin on the cell surface

FEBS Lett. 1995 Nov 13;375(1-2):11-4. doi: 10.1016/0014-5793(95)95228-o.

Abstract

Previous studies have shown that sphingolipids may be enriched in caveolae, plasmalemmal invaginations implicated in endocytosis and signal transduction. We synthesised a radiolabeled derivative of ganglioside GM1 bearing a photo-reactive cross-linker at the end of its acyl chain. After insertion in the plasma membrane of cultured A431 or MDCK cells and photoactivation, the main protein cross-linked by the GM1 derivative was VIP21-caveolin, an essential structural component of caveolae. This result shows close proximity between GM1 molecules and VIP21-caveolin in the caveolar membrane and strongly implicates sphingolipid segregation in the biogenesis of caveolae.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Autoradiography
  • Carbohydrate Sequence
  • Carrier Proteins / drug effects
  • Carrier Proteins / isolation & purification
  • Carrier Proteins / metabolism*
  • Caveolin 1
  • Caveolins*
  • Cell Line
  • Cell Membrane / metabolism
  • Cross-Linking Reagents / pharmacology*
  • Dogs
  • Electrophoresis, Gel, Two-Dimensional
  • G(M1) Ganglioside / analogs & derivatives*
  • Humans
  • Kidney
  • Membrane Proteins / drug effects
  • Membrane Proteins / isolation & purification
  • Membrane Proteins / metabolism*
  • Molecular Sequence Data
  • Tritium
  • Tumor Cells, Cultured

Substances

  • CAV1 protein, human
  • Carrier Proteins
  • Caveolin 1
  • Caveolins
  • Cross-Linking Reagents
  • Membrane Proteins
  • Tritium
  • G(M1) Ganglioside